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testing utilizing the Holm–Sidak approach with α < 0.05. Checks in which two impartial variables ended up assayed ended up analyzed employing a two-way ANOVA and Bonferroni corrections for various screening in between categories (indicated in figure legends). Remaining information were being analyzed using an unequal variance Welch t

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Only homozygous animals ended up Employed in the analyses, even though heterozygous mice had been run in behavioral experiments. 3 cohorts of eleven–sixteen mice underwent the first battery of behavioral assays for a complete of 42 animals. All mice were being originally housed with their littermates of precisely the same sex from the Vanderbilt Murine Neurobehavioral Main, with controlled 12 h gentle/dim cycles, freely obtainable food and drinking water, and managed temperature and humidity. The experimenter was in the beginning blinded towards the genotype of every animal, and each animal underwent a similar battery of behavioral checks to establish phenotype.

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